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KMID : 0376219830200030613
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Chonnam Medical Journal
1983 Volume.20 No. 3 p.613 ~ p.617
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Induction of Mutation in Salmonella typhimurium by UV Irradiation
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Abstract
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The mutagenicity of UV irradiation in salmonella tester strains of Ames was investigated by Ames plate method and a modified Ames assay adapted to the Automated Microbiology System-2 (MS-2).
TA 97, TA 98, and TA 100 strains were highly sensitive to UV light, whereas TA 102 strain was very resistant and could be used for the detection of UV-mutagenicity.
The UV irradiation (1 to 20 J/m2) produced dose-dependent increases of revertants in Ames plate test.
A modified automated salmonella mutagenicity assay using MS-2 exhibited the comparable results of UV mutagenicity in TA102 with a minimal requirement for materials and labor.
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KEYWORD
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